2018-10-28 16:05:572025-05-24 23:51:53
description
assembly of the 50S subunit of the ribosome
assembly of the 50S subunit of the [SW|ribosome]
locus
BSU16050
BSU_16050
geneLength
846
849
function
ribosome assembly
[[category|Ribosome assembly]]
outlinks
bsu
BSU16050
BSU_16050
Gene
Coordinates
1,676,532 → 1,677,380
1,676,532 1,677,380
Gene
Phenotypes of a mutant
essential [Pubmed|12682299]
depletion confers a slow growth phenotype, and induces an abnormality of the ''in vivo'' ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes [Pubmed|24335279,23700310]
premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including [[gene|rplP|L16]], [[gene|rpmA|L27]], [[gene|rpmB|L28]], [[gene|rpmGA|L33]], [[gene|rpmI|L35]] and [[gene|rpmJ|L36]] [Pubmed|24335279,23700310]
essential [Pubmed|12682299]
depletion confers a slow growth phenotype, and induces an abnormality of the ''in vivo'' [SW|ribosome] profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes [Pubmed|24335279,23700310]
premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including [[protein|RplP|L16]], [[protein|RpmA|L27]], [[protein|RpmB|L28]], [[protein|RpmGA|L33]], [[protein|RpmI|L35]] and [[protein|RpmJ|L36]] [Pubmed|24335279,23700310]
The protein
Catalyzed reaction/ biological activity
proper positioning of [[protein|RplF|L6]] in the large subunit of the [SW|ribosome] prior to incorporation of [[protein|RplP|L16]] [Pubmed|25330043]
Binds and hydrolyzes GTP and readily exchanges GDP for GTP
proper positioning of [[protein|RplF|L6]] in the large subunit of the [SW|ribosome] prior to incorporation of [[protein|RplP|L16]] [Pubmed|25330043]
Binds and hydrolyzes GTP and readily exchanges GDP for GTP
stabilizes rRNA helices in the A and P functional sites [pubmed|31665744]
The protein
Protein family
MTG1 subfamily (according to Swiss-Prot) Era/Obg family
TRAFAC class YlqF/YawG GTPase family (together with [[protein|CpgA]] and [[protein|YqeH]], according to UniProt)
The protein
[SW|Domains]
RNA-binding domain at the C-terminus [Pubmed|23325847]
RNA-binding domain at the C-terminus [Pubmed|23325847]
CP-type G domain (aa 14-178) (according to UniProt)
The protein
Structure
[PDB|1PUJ]
[PDB|1PUJ]
[PDB|6PVK] (complex of RbgA bound to ribosomal 50S subunit assembly intermediate) [pubmed|31665744]
Labs working on this gene/protein
[SW|Naotake Ogasawara], Nara, Japan
References
Original publications
labs
[SW|Naotake Ogasawara], Nara, Japan